Generation of minimal protein identifiers of proteins from two-dimensional gels and recombinant proteins. Academic Article uri icon

Overview

abstract

  • We describe the technical feasibility and methodology to characterize a protein by a minimal set of structural information generated by matrix assisted laser desorption/ionization (MALDI)-mass spectrometry, termed a "minimal protein Identifier" (MPI). MPIs can be determined for proteins from two-dimensional gels and recombinant proteins and can be used to compare and identify proteins from these sources.

authors

  • Schmidt, Frank
  • Lueking, Angelika
  • Nordhoff, Eckhard
  • Gobom, Johan
  • Klose, Joachim
  • Seitz, Harald
  • Egelhofer, Volker
  • Eickhoff, Holger
  • Lehrach, Hans
  • Cahill, Dolores J

publication date

  • February 1, 2002

Research

keywords

  • Electrophoresis, Gel, Two-Dimensional
  • Proteins
  • Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization

Identity

Scopus Document Identifier

  • 0036125469

PubMed ID

  • 11870774

Additional Document Info

volume

  • 23

issue

  • 4