LNCIB human full-length cDNAs collection: towards a better comprehension of the human transcriptome. Academic Article uri icon

Overview

abstract

  • LNCIB has been producing a variety of human full-length-enriched, normalized and subtracted cDNA libraries from various cell lines and tissues in different developmental stages by using the CAP-Trapper method. By sequencing 23000 clones of these libraries we identified a pool of about 5800 good quality unique cDNAs. After BLAST analysis on Human RefSeq/Unigene databases, 1717 of these sequences remained with no or poor annotation. We show that cross-species comparative BLAST resulted as a valid tool for the annotation of orthologous genes.

publication date

  • October 1, 2003

Research

keywords

  • Gene Library
  • Genome, Human
  • Transcription, Genetic

Identity

Scopus Document Identifier

  • 9144273280

PubMed ID

  • 14744102

Additional Document Info

volume

  • 326

issue

  • 10-11