Adeno-associated viruses containing bFGF or BDNF are neuroprotective against excitotoxicity. Academic Article uri icon

Overview

abstract

  • PURPOSE: Brain-derived neurotrophic factor (BDNF) and basic fibroblast growth factor (bFGF) hold much promise for the protection of retinal ganglion cells against excitotoxic cell death. We tested the possibility of delivering these growth factors to retinal ganglion cells via an adeno-associated viral (AAV) vector and tested their efficacy in two models of excitotoxicity. METHODS: Rat retinas were infected with AAV vectors encoding bFGF or BDNF. A control vector containing green fluorescent protein (GFP) was injected in the contralateral eye. Eyes were subjected to either an intravitreal injection of N-methyl-D-aspartate (NMDA) or optic nerve crush, and ganglion cell survival was evaluated. RESULTS: AAV.CMV.bFGF and AAV.CBA.BDNF were neuroprotective against NMDA injection 1 month post-treatment. Additionally, AAV.CMV.bFGF was protective against optic nerve crush. CONCLUSION: AAV-mediated delivery of bFGF and BDNF can promote retinal cell survival following excitotoxic insult.

publication date

  • December 1, 2004

Research

keywords

  • Brain-Derived Neurotrophic Factor
  • Dependovirus
  • Fibroblast Growth Factor 2
  • N-Methylaspartate
  • Retinal Diseases
  • Retinal Ganglion Cells

Identity

Scopus Document Identifier

  • 19944431910

Digital Object Identifier (DOI)

  • 10.1080/02713680490517872

PubMed ID

  • 15764082

Additional Document Info

volume

  • 29

issue

  • 6