Amplification of JC virus DNA from brain and cerebrospinal fluid of patients with progressive multifocal leukoencephalopathy. Academic Article uri icon

Overview

abstract

  • We have established an assay to detect JC viral DNA in brain tissue and CSF of patients with progressive multifocal leukoencephalopathy (PML) and amplified a 106-base pair segment of the gene encoding JC viral large T antigen by polymerase chain reaction (PCR) from the brains of eight patients with PML and from one of six CSF samples. Brain and CSF of patients without PML did not yield JC virus-specific DNA amplification products. Southern analysis documented that the sequences of the 106-base pair PCR product and JC viral DNA are identical. This assay can be used in the diagnosis of PML and to investigate the biology of JC virus.

publication date

  • December 1, 1991

Research

keywords

  • DNA, Viral
  • JC Virus
  • Leukoencephalopathy, Progressive Multifocal
  • Tumor Virus Infections

Identity

Scopus Document Identifier

  • 0026343609

PubMed ID

  • 1660572

Additional Document Info

volume

  • 41

issue

  • 12