No retroviremia or pathology in long-term follow-up of monkeys exposed to a murine amphotropic retrovirus. Academic Article uri icon

Overview

abstract

  • Four monkeys were exposed to a retroviral vector and replication-competent murine amphotropic retrovirus in a bone marrow transplantation/gene transfer protocol (Kantoff et al., 1987). We have studied these animals 2 and 3 years post-transplantation and did not detect replicating virus in serum, peripheral blood mononuclear cells, or bone marrow cells. Amphotropic envelope sequences could not be detected in blood or bone marrow cells by Southern blotting or the polymerase chain reaction. Antibodies directed against the p30 and gp70 viral antigens were detected by Western blot and immunoprecipitation. The animals remain alive and well. Our findings suggest that primates can clear murine amphotropic retroviruses even when exposure occurs during a time of severe immunosuppression.

publication date

  • January 1, 1991

Research

keywords

  • Genetic Engineering
  • Genetic Vectors
  • Leukemia Virus, Murine
  • Macaca fascicularis
  • Retroviridae

Identity

Scopus Document Identifier

  • 0026212584

PubMed ID

  • 1661171

Additional Document Info

volume

  • 2

issue

  • 3