Single-molecule analysis of RNA polymerase transcription. Review uri icon

Overview

abstract

  • The kinetics and mechanisms of transcription are now being investigated by a repertoire of single-molecule techniques, including optical and magnetic tweezers, high-sensitivity fluorescence techniques, and atomic force microscopy. Single-molecule techniques complement traditional biochemical and crystallographic approaches, are capable of detecting the motions and dynamics of individual RNAP molecules and transcription complexes in real time, and make it possible to directly measure RNAP binding to and unwinding of template DNA, as well as RNAP translocation along the DNA during transcript synthesis.

publication date

  • January 1, 2006

Research

keywords

  • DNA
  • DNA-Directed RNA Polymerases
  • RNA
  • Transcription, Genetic

Identity

Scopus Document Identifier

  • 33745041963

PubMed ID

  • 16689640

Additional Document Info

volume

  • 35