Molecular cloning of a human immunoglobulin heavy chain variable (VH) region with anti-myelin-associated glycoprotein activity. Academic Article uri icon

Overview

abstract

  • A cDNA clone that encodes the heavy chain variable region (VH) of an IgM M-protein with anti-myelin-associated glycoprotein (MAG) activity secreted by chronic lymphocytic leukemia cells (B-C11) from a patient with peripheral neuropathy was cloned and sequenced. The JH region was identical to the germline JH4 sequence except for deletion of a thymidine residue at the site of D-JH recombination, and the D region showed greatest homology to DM2. Sequence analysis of the VH region revealed greatest homology to VH26, a member of the VH3 gene family, but homology was only 83.7% over 326 bases, suggesting that it was derived from as yet an unidentified member of the VH3 gene family.

publication date

  • January 1, 1990

Research

keywords

  • Antibodies
  • Cloning, Molecular
  • Immunoglobulin Heavy Chains
  • Myelin Proteins

Identity

Scopus Document Identifier

  • 0025004936

PubMed ID

  • 1688442

Additional Document Info

volume

  • 26

issue

  • 1