Global proteome profiling of NPM/ALK-positive anaplastic large cell lymphoma. Academic Article uri icon

Overview

abstract

  • OBJECTIVE: Constitutive overexpression of nucleophosmin/anaplastic lymphoma kinase (NPM/ALK) is a key oncogenic event in anaplastic large cell lymphomas (ALCL) that carry the t(2;5)(p23;q35) translocation. Global proteomic analysis of NPM/ALK-positive ALCL would improve understanding of the disease pathogenesis and yield new candidate targets for novel treatment and diagnostic strategies. MATERIALS AND METHODS: To comprehensively determine the inventory of proteins from NPM/ALK-positive ALCL SUDHL-1 cells, the membrane, cytoplasm, and nuclear subcellular fractions were resolved by one-dimensional sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and analyzed using liquid chromatography-tandem mass spectrometry (LC-MS/MS). The MS spectra were interpreted using SEQUEST to search the electronic UniProt protein database, and analyzed by ProteinProphet and INTERACT. RESULTS: A total of 623 proteins consisting of 210 membrane, 229 cytoplasm, and 184 nuclear proteins were identified with a

publication date

  • June 7, 2007

Research

keywords

  • Gene Expression Profiling
  • Lymphoma, Large B-Cell, Diffuse
  • Oncogene Proteins, Fusion
  • Protein-Tyrosine Kinases
  • Proteome

Identity

Scopus Document Identifier

  • 34447619666

Digital Object Identifier (DOI)

  • 10.1016/j.exphem.2007.04.011

PubMed ID

  • 17560012

Additional Document Info

volume

  • 35

issue

  • 8