A transgenic mouse model for high content, cell cycle phenotype screening in live primary cells. Academic Article uri icon

Overview

abstract

  • High content cell-based genetic and small molecule library screens are powerful strategies in drug discovery and investigations of disease mechanisms. We report that primary cells derived from a transgenic mouse model expressing a fluorescence mitosis biosensor provide unambiguous phenotype readouts without the need for transfection or immunocytochemistry. Phenotype profiles of cell cycle disruption and of apoptosis are easily detectable at a single time point selected from time-lapse live fluorescence microscopy. Most importantly, this transgenic mouse model may be crossed with cancer mouse models to derive biosensor-expressing primary cancer cells for use in high content screening strategies targeting discovery of tumor-specific chemotherapeutic compounds.

publication date

  • July 9, 2007

Research

keywords

  • Cell Cycle
  • Fibroblasts
  • Genetic Testing
  • Models, Animal
  • Phenotype

Identity

Scopus Document Identifier

  • 35148854925

PubMed ID

  • 17881898

Additional Document Info

volume

  • 6

issue

  • 18