Immunostimulatory oligodeoxynucleotide containing TTTCGTTT motif from Lactobacillus rhamnosus GG DNA potentially suppresses OVA-specific IgE production in mice. Academic Article uri icon

Overview

abstract

  • In a previous study of the immunoregulatory properties of commensal bacterial DNA, we identified the strong immunostimulatory oligodeoxynucleotide (ISS-ODN) ID35 in the genomic DNA of Lactobacillus rhamnosus GG (LGG). The observed effects of ID35 are because of the unique TTTCGTTT motif located at the 5' end of the ODN, which is different from the previously identified ISS motifs in humans and mice. In the present study, we used an ovalbumin (OVA)-sensitized mouse model to show that ID35 is a potent suppressor of antigen-specific immunoglobulin E (IgE) production in vivo. This effect was toll-like receptor 9-dependent, as GpC negID35 failed to suppress antigen-specific IgE production. ID35 activated the specific subset of CD11c+CD8a+ dendritic cells, which are associated with T-helper 1 (Th1)-type systemic responses, and effectively induced interferon-gamma (IFN-gamma) production by CD4+ T cells in OVA-challenged mice. These immunoregulatory effects of ID35 were comparable with those induced by the murine prototype ODN 1826. Thus, ID35 is the first ISS-ODN with such a strong immunostimulatory and IgE suppressor activity to be found in immunobiotic bacterial DNA.

publication date

  • February 1, 2008

Research

keywords

  • Allergens
  • Hypersensitivity
  • Immunoglobulin E
  • Immunosuppression
  • Immunosuppression Therapy
  • Lacticaseibacillus rhamnosus
  • Lactobacillus rhamnosus
  • Oligodeoxyribonucleotides
  • Ovalbumin

Identity

Scopus Document Identifier

  • 40849098657

Digital Object Identifier (DOI)

  • 10.1111/j.1365-3083.2008.02080.x

PubMed ID

  • 18248528

Additional Document Info

volume

  • 67

issue

  • 4