Rapid isolation and identification of bacteriophage T4-encoded modifications of Escherichia coli RNA polymerase: a generic method to study bacteriophage/host interactions. Academic Article uri icon

Overview

abstract

  • Bacteriophages are bacterial viruses that infect bacterial cells, and they have developed ingenious mechanisms to modify the bacterial RNA polymerase. Using a rapid, specific, single-step affinity isolation procedure to purify Escherichia coli RNA polymerase from bacteriophage T4-infected cells, we have identified bacteriophage T4-dependent modifications of the host RNA polymerase. We suggest that this methodology is broadly applicable for the identification of bacteriophage-dependent alterations of the host synthesis machinery.

authors

  • Westblade, Lars F
  • Minakhin, Leonid
  • Kuznedelov, Konstantin
  • Tackett, Alan J
  • Chang, Emmanuel J
  • Mooney, Rachel A
  • Vvedenskaya, Irina
  • Wang, Qing Jun
  • Fenyƶ, David
  • Rout, Michael P
  • Landick, Robert
  • Chait, Brian T
  • Severinov, Konstantin
  • Darst, Seth A

publication date

  • February 14, 2008

Research

keywords

  • Bacteriophage T4
  • DNA-Directed RNA Polymerases
  • Escherichia coli

Identity

PubMed Central ID

  • PMC2612130

Scopus Document Identifier

  • 44449102824

Digital Object Identifier (DOI)

  • 10.1021/pr070451j

PubMed ID

  • 18271525

Additional Document Info

volume

  • 7

issue

  • 3