Design of a fluorescence polarization assay platform for the study of human Hsp70. Academic Article uri icon

Overview

abstract

  • The 70kDa heat shock proteins (Hsp70) are molecular chaperones that assist in folding of newly synthesized polypeptides, refolding or denaturation of misfolded proteins, and translocation of proteins across biological membranes. In addition, Hsp70 play regulatory roles in signal transduction, cell cycle, and apoptosis. Here, we present a novel assay platform based on fluorescence polarization that is suitable for investigating the yet elusive molecular mechanics of human Hsp70 allosteric regulation.

publication date

  • May 16, 2008

Research

keywords

  • Fluorescence Polarization
  • Fluorescent Dyes
  • HSP70 Heat-Shock Proteins
  • Spectrometry, Fluorescence

Identity

PubMed Central ID

  • PMC2538440

Scopus Document Identifier

  • 44849097137

Digital Object Identifier (DOI)

  • 10.1016/j.bmcl.2008.05.046

PubMed ID

  • 18515098

Additional Document Info

volume

  • 18

issue

  • 13