Suitability of silica hydride stationary phase, aqueous normal phase chromatography for untargeted metabolomic profiling of Enterococcus faecium and Staphylococcus aureus. Academic Article uri icon

Overview

abstract

  • We report the robustness of silica hydride stationary phase, aqueous normal phase (ANP) chromatography to the chemical complexity of the intracellular metabolomes of Staphylococcus aureus and Enterococcus faecium. We specifically demonstrate that the chromatographic behavior of known metabolites is unaffected by the intracellular chemical matrix of these microbes and that this method enables untargeted profiling of their intracellular metabolites using accurate mass-retention time (AMRT) identifiers. We further demonstrate the ability of AMRT-based metabolite profiling to differentiate bacteria along genetic and phenotypic lines. Overall, these data commend the utility of ANP-based chromatography for untargeted metabolomics-based studies of microbial physiology and antibiotic resistance.

publication date

  • July 1, 2009

Research

keywords

  • Chromatography
  • Enterococcus faecium
  • Silicates
  • Staphylococcus aureus
  • Water

Identity

PubMed Central ID

  • PMC5821128

Scopus Document Identifier

  • 70149098295

Digital Object Identifier (DOI)

  • 10.1002/jssc.200900256

PubMed ID

  • 19569105

Additional Document Info

volume

  • 32

issue

  • 13