Limiting false-positive polymerase chain reaction results: detection of DNA and mRNA to differentiate viable from dead bacteria. Academic Article uri icon

Overview

abstract

  • We compared 2 methods for determining Escherichia coli viability in vitro. A 16S rDNA polymerase chain reaction (PCR) assay detected bacteria irrespective of viability. A groEL mRNA reverse transcriptase PCR was positive for 72 h but later became negative. Detecting mRNA holds promise but is tedious, and groEL may not be the best target.

publication date

  • August 1, 2009

Research

keywords

  • Bacterial Infections
  • DNA, Bacterial
  • Diagnostic Errors
  • Microbial Viability
  • Polymerase Chain Reaction
  • RNA, Bacterial
  • RNA, Messenger

Identity

Scopus Document Identifier

  • 67650714196

Digital Object Identifier (DOI)

  • 10.1016/j.diagmicrobio.2009.04.004

PubMed ID

  • 19631099

Additional Document Info

volume

  • 64

issue

  • 4