Labeling human mesenchymal stem cells with fluorescent contrast agents: the biological impact. Academic Article uri icon



  • PURPOSE: This study aims to determine the effect of human mesenchymal stem cell (hMSC) labeling with the fluorescent dye DiD and the iron oxide nanoparticle ferucarbotran on chondrogenesis. PROCEDURES: hMSCs were labeled with DiD alone or with DiD and ferucarbotran (DiD/ferucarbotran). hMSCs underwent confocal microscopy, optical imaging (OI), and magnetic resonance (MR) imaging. Chondrogenesis was induced by transforming growth factor-b and confirmed by histopathology and glycosaminoglycan (GAG) production. Data of labeled and unlabeled hMSCs were compared with a t test. RESULTS: Cellular uptake of DiD and ferucarbotran was confirmed with confocal microscopy. DiD labeling caused a significant fluorescence on OI, and ferucarbotran labeling caused a significant T2* effect on MR images. Compared to nonlabeled controls, progenies of labeled MSCs exhibited similar chondrocyte morphology after chondrogenic differentiation, but the labeled cells demonstrated significantly reduced GAG production (p < 0.05). CONCLUSION: DiD and DiD/ferucarbotran labeling of hMSC does not interfere with cell viability or morphologic differentiation into chondrocytes, but labeled cells exhibit significantly less GAG production compared to unlabeled cells.

publication date

  • February 1, 2011



  • Contrast Media
  • Fluorescent Dyes
  • Mesenchymal Stem Cells


PubMed Central ID

  • PMC3023037

Scopus Document Identifier

  • 79551613094

Digital Object Identifier (DOI)

  • 10.1007/s11307-010-0322-0

PubMed ID

  • 20379785

Additional Document Info


  • 13


  • 1