Deep sequencing of the small RNA transcriptome of normal and malignant human B cells identifies hundreds of novel microRNAs. Academic Article uri icon

Overview

abstract

  • A role for microRNA (miRNA) has been recognized in nearly every biologic system examined thus far. A complete delineation of their role must be preceded by the identification of all miRNAs present in any system. We elucidated the complete small RNA transcriptome of normal and malignant B cells through deep sequencing of 31 normal and malignant human B-cell samples that comprise the spectrum of B-cell differentiation and common malignant phenotypes. We identified the expression of 333 known miRNAs, which is more than twice the number previously recognized in any tissue type. We further identified the expression of 286 candidate novel miRNAs in normal and malignant B cells. These miRNAs were validated at a high rate (92%) using quantitative polymerase chain reaction, and we demonstrated their application in the distinction of clinically relevant subgroups of lymphoma. We further demonstrated that a novel miRNA cluster, previously annotated as a hypothetical gene LOC100130622, contains 6 novel miRNAs that regulate the transforming growth factor-β pathway. Thus, our work suggests that more than a third of the miRNAs present in most cellular types are currently unknown and that these miRNAs may regulate important cellular functions.

authors

publication date

  • August 23, 2010

Research

keywords

  • B-Lymphocytes
  • Gene Expression Profiling
  • Lymphoma, Large B-Cell, Diffuse
  • MicroRNAs

Identity

PubMed Central ID

  • PMC3012600

Scopus Document Identifier

  • 78649743127

Digital Object Identifier (DOI)

  • 10.1182/blood-2010-05-285403

PubMed ID

  • 20733160

Additional Document Info

volume

  • 116

issue

  • 23