Activation of MKK3/6, SAPK, and ATF-2/c-jun in ACL fibroblasts grown in 3 dimension collagen gels in response to application of cyclic strain. Academic Article uri icon

Overview

abstract

  • Signal transduction pathways involved in response to cyclic tensile strain and strain deprivation in anterior cruciate ligament (ACL) fibroblasts grown in 3D collagen gels were investigated. Application of cyclic tensile strain resulted in significant activation (phosphorylation) of MKK3/6, SAPK and their downstream target transcription factors, ATF-2 and c-jun, while strain deprivation resulted in a decrease in these kinases and transcription factors. These data suggest that ACL fibroblasts cultured in 3D collagen gels respond to the mechanical environment and provide a useful system for determination of the molecular mechanisms involved in the regulation of proliferation and matrix turnover by mechanical load.

publication date

  • September 30, 2010

Research

keywords

  • Activating Transcription Factor 2
  • Fibroblasts
  • MAP Kinase Kinase 3
  • MAP Kinase Kinase 6
  • MAP Kinase Signaling System
  • Mitogen-Activated Protein Kinase 8

Identity

Scopus Document Identifier

  • 79251582218

Digital Object Identifier (DOI)

  • 10.1002/jor.21244

PubMed ID

  • 20886655

Additional Document Info

volume

  • 29

issue

  • 3