Assessment of cardiovascular fibrosis using novel fluorescent probes. Academic Article uri icon

Overview

abstract

  • Cardiovascular fibrosis resulted from pressure overload or ischemia could alter myocardial stiffness and lead to ventricular dysfunction. Fluorescently labeled collagen-binding protein CNA 35, derived from the surface component of Staphylococcus aureus, and a novel synthetic biphenylalanine containing peptide are applied to stain fibrosis associated collagen and myocytes, respectively. Detailed pathological characteristics of cardiovascular fibrosis could be identified clearly in 2 hours. This staining pair requires only simple staining and brief washing, generating less than 10 ml of waste. The image information collected by this novel fluorescent staining pair is compatible with it collected by the traditional Masson's Trichrome and Picrosirius Red staining which are widely used to stain cardiovascular fibrosis and isolated cells.

publication date

  • April 20, 2011

Research

keywords

  • Cardiovascular Diseases
  • Fluorescent Dyes

Identity

PubMed Central ID

  • PMC3080412

Scopus Document Identifier

  • 79955377033

Digital Object Identifier (DOI)

  • 10.1371/journal.pone.0019097

PubMed ID

  • 21533060

Additional Document Info

volume

  • 6

issue

  • 4