Co-transplantation of fetal bone tissue facilitates the development and reconstitution in human B cells in humanized NOD/SCID/IL-2Rγnull (NSG) mice. Academic Article uri icon

Overview

abstract

  • BACKGROUND: In terms of the function and reconstitution efficacy of human immune cells, co-transplantation of human fetal tissues, such as thymus and liver, with CD34(+) hematopoietic stem cells (HSCs) has potential advantages in the generation of humanized mice. OBJECTIVE AND METHODS: To examine the effects of bone tissues in the reconstitution of human immune cells, particularly in B cells, we generated a new humanized mice co-transplanted with human fetal thymus (hFT)/fetal bone (hFB) tissues and human fetal liver-derived CD34(+) cells. RESULTS: Humanized mice exhibited effective reconstitution of human immune cells earlier compared to control humanized mice. In terms of quantity, the number of immune cells, such as human T, B, and monocyte/macrophages was significantly increased. Furthermore, significant increase of B cell progenitors and immature/naïve B cells could be detected in the bone marrow and spleen of humanized mice. CONCLUSION: Our results demonstrate that co-transplantation of hFB tissue may facilitate the reconstitution of human B and T cells, and therefore the humanized model may be used to develop therapeutic human antibodies for clinical use.

authors

  • Kim, SoYoung
  • Kim, Miyoung
  • Choi, Bongkum
  • Kim, So Yong
  • Yang, Ji-Hyuk
  • Roh, Cheong Rae
  • Lee, Ki-Young
  • Kim, Sung Joo

publication date

  • May 5, 2011

Research

keywords

  • B-Lymphocytes
  • Bone Transplantation
  • Bone and Bones
  • Hematopoietic Stem Cell Transplantation
  • Thymus Gland
  • Transplantation, Heterologous

Identity

Scopus Document Identifier

  • 80555155583

Digital Object Identifier (DOI)

  • 10.1007/s10875-011-9538-2

PubMed ID

  • 21544592

Additional Document Info

volume

  • 31

issue

  • 4