Turnover of ATP synthase subunits in F1-depleted HeLa and yeast cells. Academic Article uri icon

Overview

abstract

  • Mitochondrial translation of the Saccharomyces cerevisiae Atp6p subunit of F(1)-F(0) ATP synthase is regulated by the F(1) ATPase. Here we show normal expression of Atp6p in HeLa cells depleted of the F(1) β subunit. Instead of being translationally down-regulated, HeLa cells lacking F(1) degrade Atp6p, thereby preventing proton leakage across the inner membrane. Mammalian mitochondria also differ in the way they minimize the harmful effect of unassembled F(1) α subunit. While yeast mutants lacking β subunit have stable aggregated F(1) α subunit in the mitochondrial matrix, the human α subunit is completely degraded in cells deficient in F(1) β subunit. These results are discussed in light of the different properties of the proteins and environments in which yeast and human mitochondria exist.

publication date

  • July 23, 2011

Research

keywords

  • Mitochondrial Proton-Translocating ATPases
  • Protein Subunits
  • Saccharomyces cerevisiae

Identity

PubMed Central ID

  • PMC3157936

Scopus Document Identifier

  • 80051794191

Digital Object Identifier (DOI)

  • 10.1016/j.febslet.2011.07.011

PubMed ID

  • 21784071

Additional Document Info

volume

  • 585

issue

  • 16