The Candida albicans Ku70 modulates telomere length and structure by regulating both telomerase and recombination. Academic Article uri icon

Overview

abstract

  • The heterodimeric Ku complex has been shown to participate in DNA repair and telomere regulation in a variety of organisms. Here we report a detailed characterization of the function of Ku70 in the diploid fungal pathogen Candida albicans. Both ku70 heterozygous and homozygous deletion mutants have a wild-type colony and cellular morphology, and are not sensitive to MMS or UV light. Interestingly, we observed complex effects of KU70 gene dosage on telomere lengths, with the KU70/ku70 heterozygotes exhibiting slightly shorter telomeres, and the ku70 null strain exhibiting long and heterogeneous telomeres. Analysis of combination mutants suggests that the telomere elongation in the ku70 null mutant is due mostly to unregulated telomerase action. In addition, elevated levels of extrachromosomal telomeric circles were detected in the null mutant, consistent with activation of aberrant telomeric recombination. Altogether, our observations point to multiple mechanisms of the Ku complex in telomerase regulation and telomere protection in C. albicans, and reveal interesting similarities and differences in the mechanisms of the Ku complex in disparate systems.

publication date

  • August 23, 2011

Research

keywords

  • Antigens, Nuclear
  • Candida albicans
  • DNA-Binding Proteins
  • Recombination, Genetic
  • Telomerase
  • Telomere

Identity

PubMed Central ID

  • PMC3160324

Scopus Document Identifier

  • 80051978815

Digital Object Identifier (DOI)

  • 10.1371/journal.pone.0023732

PubMed ID

  • 21886818

Additional Document Info

volume

  • 6

issue

  • 8