Evaluation of eIF4E expression in an osteosarcoma-specific tissue microarray. Academic Article uri icon

Overview

abstract

  • The ability to define osteosarcoma (OS) patients at greatest risk for metastatic progression and nonresponsiveness to conventional therapy is currently not possible. Such biomarkers are needed to predict overall prognosis, probability of metastases at diagnosis, and response to chemotherapy. The tissue microarray (TMA) serves as a powerful tool for detecting and validating protein biomarkers across a variety of patients. We constructed a novel outcome-linked TMA to add to and address shortcomings of currently available OS tissue resources. To test the use of our TMA, we surveyed the expression of eukaryotic initiation factor 4E (eIF4E) in OS patients using immunohistochemistry. Aberrant regulation of translation initiation is a feature of many cancers. eIF4E is central to initiation of protein synthesis. Its expression and activity have been implicated in tumor formation and potentially malignant and/or metastatic progression in some carcinomas. We found that eIF4E was uniformly expressed in OS patient samples. No association was found between eIF4E and outcome in OS patients. This novel OS TMA provided a facile mechanism to assess the role of a relevant protein biomarker in OS.

publication date

  • October 1, 2011

Research

keywords

  • Biomarkers, Tumor
  • Bone Neoplasms
  • Eukaryotic Initiation Factor-4E
  • Osteosarcoma
  • Tissue Array Analysis

Identity

PubMed Central ID

  • PMC3179611

Scopus Document Identifier

  • 80053321287

Digital Object Identifier (DOI)

  • 10.1097/MPH.0b013e318223d0c1

PubMed ID

  • 21941146

Additional Document Info

volume

  • 33

issue

  • 7