Multiplex real-time PCR for detection of an epidemic KPC-producing Klebsiella pneumoniae ST258 clone. Academic Article uri icon

Overview

abstract

  • We describe a multiplex real-time PCR assay capable of identifying both the epidemic Klebsiella pneumoniae ST258 clone and bla(KPC) carbapenemase genes in a single reaction. The assay displayed excellent sensitivity (100%) and specificity (100%) for identification of ST258 clone and bla(KPC) in a collection of 75 K. pneumoniae isolates comprising 41 sequence types. Our results suggest that this assay is an effective tool for surveillance of this clone among carbapenem-resistant K. pneumoniae clinical isolates.

publication date

  • March 26, 2012

Research

keywords

  • Bacterial Proteins
  • Klebsiella pneumoniae
  • Real-Time Polymerase Chain Reaction
  • beta-Lactamases

Identity

PubMed Central ID

  • PMC3370784

Scopus Document Identifier

  • 84861146913

Digital Object Identifier (DOI)

  • 10.1128/AAC.00316-12

PubMed ID

  • 22450983

Additional Document Info

volume

  • 56

issue

  • 6