Potential problems in the use of commercial preparations of radiolabeled cholesterol. Academic Article uri icon

Overview

abstract

  • Through a series of biological and analytical procedures, we demonstrate that a compound purchased from a commercial supplier as [7-3H]cholesterol was not cholesterol. In mouse peritoneal macrophages, this compound was metabolized differently than other radiolabeled cholesterol preparations and was accumulated in the steryl ester pool. In contrast, Fu5AH rat hepatoma cells did not discriminate this compound from cholesterol. Further analysis of the anomalous [7-3H]cholesterol by TLC after cholesterol oxidase treatment and by HPLC indicated that this radiochemical was less polar than cholesterol standard and other radiolabeled cholesterol preparations tested. Mass spectrometry analysis disclosed that the chemical has a similar fragmentation pattern and the same molecular weight (386) as cholesterol.

publication date

  • October 1, 1990

Research

keywords

  • Cholesterol
  • Tritium

Identity

Scopus Document Identifier

  • 0025012343

PubMed ID

  • 2282104

Additional Document Info

volume

  • 84

issue

  • 2-3