Uptake of silica and carbon nanotubes by human macrophages/monocytes induces activation of fibroblasts in vitro -- potential implication for pathogenesis of inflammation and fibrotic diseases. Academic Article uri icon

Overview

abstract

  • The potential pathogenic effects of silica and carbon nanotubes (CNTs) on fibroblasts, macrophages/monocytes, and T cells were investigated. Human macrophage/monocytes were cultured and stimulated with silica, CNTs, or titanium particles. After adding human T cells to the stimulated macrophages/monocytes, the cells were added to cultured human fibroblasts. Upon microscopic examination, CNT stimulation after 24 hours showed centralization of macrophages/monocytes around the CNTs. Silica stimulation showed a significant increase of IL-1α and IL-1β in cultured medium, and an increased gene expression of CTGF in cultured fibroblasts at 1 hour, as well as an up-regulation of the COL1A2 gene at 24-hour time point. In addition to the same changes of IL-1α, IL-1β and the COL1A2 by silica, CNT stimulation showed an increase of IL-8 in cultured medium at 1-hour time point. Titanium stimulation yielded no significant changes. The results indicate a proinflammatory and/or profibrotic effect of silica and CNTs to cultured human cells including macrophages/monocyte, T cells and fibroblasts.

publication date

  • July 1, 2012

Research

keywords

  • Cell Communication
  • Fibroblasts
  • Inflammation
  • Macrophages
  • Nanotubes, Carbon
  • Silicon Dioxide

Identity

Scopus Document Identifier

  • 84868006730

PubMed ID

  • 23058021

Additional Document Info

volume

  • 25

issue

  • 3