Quantitation of HIV-1 DNA with a sensitive TaqMan assay that has broad subtype specificity. Academic Article uri icon

Overview

abstract

  • The increasing diversity of HIV-1 isolates makes virus quantitation challenging, especially when diverse isolates co-circulate in a geographical area. Measuring the HIV-1 DNA levels in cells has become a valuable practical tool for fundamental and clinical research. A quantitative HIV-1 DNA assay was developed based on TaqMan(®) technology. Primers that target the highly conserved LTR region were designed to detect a broad array of HIV-1 variants, including viral isolates from many subtypes, with high sensitivity. Introduction of a pre-amplification step prior to the TaqMan(®) reaction allowed the specific amplification of fully reverse transcribed viral DNA. Execution of the pre-amplification step with a second primer set enables for the exclusive quantitation of the 2-LTR circular HIV-1 DNA form.

publication date

  • October 8, 2012

Research

keywords

  • DNA, Viral
  • HIV-1
  • Polymerase Chain Reaction

Identity

Scopus Document Identifier

  • 84871729888

Digital Object Identifier (DOI)

  • 10.1016/j.jviromet.2012.09.019

PubMed ID

  • 23059551

Additional Document Info

volume

  • 187

issue

  • 1