Studying cancer stem cell dynamics on PDMS surfaces for microfluidics device design. Academic Article uri icon

Overview

abstract

  • This systematic study clarified a few interfacial aspects of cancer cell phenotypes on polydimethylsiloxane (PDMS) substrates and indicated that the cell phenotypic equilibrium greatly responds to cell-to-surface interactions. We demonstrated that coatings of fibronectin, bovine serum albumin (BSA), or collagen with or without oxygen-plasma treatments of the PDMS surfaces dramatically impacted the phenotypic equilibrium of breast cancer stem cells, while the variations of the PDMS elastic stiffness had much less such effects. Our results showed that the surface coatings of collagen and fibronectin on PDMS maintained breast cancer cell phenotypes to be nearly identical to the cultures on commercial polystyrene Petri dishes. The surface coating of BSA provided a weak cell-substrate adhesion that stimulated the increase in stem-cell-like subpopulation. Our observations may potentially guide surface modification approaches to obtain specific cell phenotypes.

publication date

  • January 1, 2013

Research

keywords

  • Coated Materials, Biocompatible
  • Dimethylpolysiloxanes
  • Microfluidic Analytical Techniques
  • Neoplastic Stem Cells
  • Tissue Engineering

Identity

PubMed Central ID

  • PMC3728601

Scopus Document Identifier

  • 84881417541

Digital Object Identifier (DOI)

  • 10.1038/srep02332

PubMed ID

  • 23900274

Additional Document Info

volume

  • 3