Metabolic characterization of a Sirt5 deficient mouse model. Academic Article uri icon

Overview

abstract

  • Sirt5, localized in the mitochondria, is a member of sirtuin family of NAD⁺-dependent deacetylases. Sirt5 was shown to deacetylate and activate carbamoyl phosphate synthase 1. Most recently, Sirt5 was reported to be the predominant protein desuccinylase and demalonylase in the mitochondria because the ablation of Sirt5 enhanced the global succinylation and malonylation of mitochondrial proteins, including many metabolic enzymes. In order to determine the physiological role of Sirt5 in metabolic homeostasis, we generated a germline Sirt5 deficient (Sirt5⁻/⁻) mouse model and performed a thorough metabolic characterization of this mouse line. Although a global protein hypersuccinylation and elevated serum ammonia during fasting were observed in our Sirt5⁻/⁻ mouse model, Sirt5 deficiency did not lead to any overt metabolic abnormalities under either chow or high fat diet conditions. These observations suggest that Sirt5 is likely to be dispensable for the metabolic homeostasis under the basal conditions.

publication date

  • September 30, 2013

Research

keywords

  • Metabolome
  • Sirtuins

Identity

PubMed Central ID

  • PMC3786297

Scopus Document Identifier

  • 84885124677

Digital Object Identifier (DOI)

  • 10.1038/srep02806

PubMed ID

  • 24076663

Additional Document Info

volume

  • 3