HSPH1 inhibition downregulates Bcl-6 and c-Myc and hampers the growth of human aggressive B-cell non-Hodgkin lymphoma. Academic Article uri icon



  • We have shown that human B-cell non-Hodgkin lymphomas (B-NHLs) express heat shock protein (HSP)H1/105 in function of their aggressiveness. Here, we now clarify its role as a functional B-NHL target by testing the hypothesis that it promotes the stabilization of key lymphoma oncoproteins. HSPH1 silencing in 4 models of aggressive B-NHLs was paralleled by Bcl-6 and c-Myc downregulation. In vitro and in vivo analysis of HSPH1-silenced Namalwa cells showed that this effect was associated with a significant growth delay and the loss of tumorigenicity when 10(4) cells were injected into mice. Interestingly, we found that HSPH1 physically interacts with c-Myc and Bcl-6 in both Namalwa cells and primary aggressive B-NHLs. Accordingly, expression of HSPH1 and either c-Myc or Bcl-6 positively correlated in these diseases. Our study indicates that HSPH1 concurrently favors the expression of 2 key lymphoma oncoproteins, thus confirming its candidacy as a valuable therapeutic target of aggressive B-NHLs.


  • Zappasodi, Roberta
  • Ruggiero, Giusi
  • Guarnotta, Carla
  • Tortoreto, Monica
  • Tringali, Cristina
  • Cavanè, Alessandra
  • Cabras, Antonello D
  • Castagnoli, Lorenzo
  • Venerando, Bruno
  • Zaffaroni, Nadia
  • Gianni, Alessandro M
  • De Braud, Filippo
  • Tripodo, Claudio
  • Pupa, Serenella M
  • Di Nicola, Massimo

publication date

  • January 8, 2015



  • DNA-Binding Proteins
  • HSP110 Heat-Shock Proteins
  • Lymphoma, B-Cell
  • Proto-Oncogene Proteins c-myc


Scopus Document Identifier

  • 84924969034

Digital Object Identifier (DOI)

  • 10.1182/blood-2014-07-590034

PubMed ID

  • 25573990

Additional Document Info


  • 125


  • 11