Conditions for (13)C NMR detection of 2-hydroxyglutarate in tissue extracts from isocitrate dehydrogenase-mutated gliomas. Academic Article uri icon

Overview

abstract

  • (13)C NMR (nuclear magnetic resonance) spectroscopy of extracts from patient tumor samples provides rich information about metabolism. However, in isocitrate dehydrogenase (IDH)-mutant gliomas, (13)C labeling is obscured in oncometabolite 2-hydroxyglutaric acid (2 HG) by glutamate and glutamine, prompting development of a simple method to resolve the metabolites. J-coupled multiplets in 2 HG were similar to glutamate and glutamine and could be clearly resolved at pH 6. A cryogenically cooled (13)C probe, but not J-resolved heteronuclear single quantum coherence spectroscopy, significantly improved detection of 2 HG. These methods enable the monitoring of (13)C-(13)C spin-spin couplings in 2 HG expressing IDH-mutant gliomas.

authors

  • Pichumani, Kumar
  • Mashimo, Tomoyuki
  • Baek, Hyeon-Man
  • Ratnakar, James
  • Mickey, Bruce
  • DeBerardinis, Ralph J
  • Maher, Elizabeth A
  • Bachoo, Robert M
  • Malloy, Craig R
  • Kovacs, Zoltan

publication date

  • April 20, 2015

Research

keywords

  • Glioma
  • Glutarates
  • Isocitrate Dehydrogenase
  • Magnetic Resonance Spectroscopy

Identity

PubMed Central ID

  • PMC4458399

Scopus Document Identifier

  • 84930277052

Digital Object Identifier (DOI)

  • 10.1016/j.ab.2015.04.017

PubMed ID

  • 25908561

Additional Document Info

volume

  • 481