Derivation of Diverse Hormone-Releasing Pituitary Cells from Human Pluripotent Stem Cells. Academic Article uri icon

Overview

abstract

  • Human pluripotent stem cells (hPSCs) provide an unlimited cell source for regenerative medicine. Hormone-producing cells are particularly suitable for cell therapy, and hypopituitarism, a defect in pituitary gland function, represents a promising therapeutic target. Previous studies have derived pituitary lineages from mouse and human ESCs using 3D organoid cultures that mimic the complex events underlying pituitary gland development in vivo. Instead of relying on unknown cellular signals, we present a simple and efficient strategy to derive human pituitary lineages from hPSCs using monolayer culture conditions suitable for cell manufacturing. We demonstrate that purified placode cells can be directed into pituitary fates using defined signals. hPSC-derived pituitary cells show basal and stimulus-induced hormone release in vitro and engraftment and hormone release in vivo after transplantation into a murine model of hypopituitarism. This work lays the foundation for future cell therapy applications in patients with hypopituitarism.

publication date

  • June 14, 2016

Research

keywords

  • Corticotrophs
  • Embryonic Stem Cells
  • Hypopituitarism
  • Pluripotent Stem Cells
  • Thyrotrophs

Identity

PubMed Central ID

  • PMC4912387

Scopus Document Identifier

  • 84975229970

Digital Object Identifier (DOI)

  • 10.1016/j.stemcr.2016.05.005

PubMed ID

  • 27304916

Additional Document Info

volume

  • 6

issue

  • 6