Localization of surface vWF on resting and stimulated platelets. Academic Article uri icon

Overview

abstract

  • We used immunoelectron microscopic localization techniques to investigate whether platelets stimulated by ADP or ristocetin in the plasma milieu bind von Willebrand factor (vWF) to their surfaces. We found by both peroxidase- and ferritin-based methods that unstimulated platelets lack vWF on their surfaces, whereas platelets that are stimulated with ADP or ristocetin have vWF associated with their surfaces. The specificity of the findings was confirmed by absorption studies using severe von Willebrand disease (vWD) and hemophilic plasmas. The anti-vWF antibodies were blocked by incubation with hemophilic plasma but not by incubation with severe vWD plasma. Thus, in the plasma environment, in the presence of fibrinogen, vWF becomes associated with the platelet surface subsequent to stimulation with ADP or ristocetin.

publication date

  • November 1, 1987

Research

keywords

  • Blood Platelets
  • Platelet Membrane Glycoproteins
  • Receptors, Cell Surface
  • von Willebrand Factor

Identity

Scopus Document Identifier

  • 0023572227

PubMed ID

  • 2822169

Additional Document Info

volume

  • 70

issue

  • 5