Sensitive ELISA for the gp120 and gp160 surface glycoproteins of HIV-1. Academic Article uri icon

Overview

abstract

  • We have used a panel of polyclonal and monoclonal antibodies against gp120 and gp160, the envelope glycoproteins of human immunodeficiency virus type 1, to create rapid, simple, and sensitive twin-site sandwich ELISA specific for gp120 and gp160 or for gp160 alone. These assays can detect 500 COS cells in a population transiently transfected with a construct encoding gp120 and gp160, or 50 pg of recombinant gp160. We estimate that the mean amount of gp120 + gp160 in the transfected population is equivalent to 2.5 x 10(6) molecules per cell, 40-50% of which can be recovered from the culture medium as gp120 after 24 hours. The ELISA can be adapted to assess whether gp120 is detectable in the sera of HIV-1-infected persons: we show that gp120/gp160 is completely stable in normal human serum for at least 24 hours at 37 degrees C.

publication date

  • October 1, 1988

Research

keywords

  • Enzyme-Linked Immunosorbent Assay
  • HIV
  • Retroviridae Proteins
  • Viral Envelope Proteins

Identity

Scopus Document Identifier

  • 0023790793

PubMed ID

  • 2848557

Additional Document Info

volume

  • 4

issue

  • 5