Evaluation of a human adenovirus viral load assay using the Altona RealStar® PCR test. Academic Article uri icon

Overview

abstract

  • This study evaluated the performance of the Altona Diagnostics RealStar® Adenovirus Research Use Only (RUO) real-time PCR reagents for HAdV quantitation in plasma samples from immunodeficient patients. The assay was linear from 2.30-9.17 log10 copies/mL (coefficient of determination; R2=0.998) with limits of detection and quantification of 2.19 log10 and 2.30 log10 copies/mL (>95% positivity rate), respectively. Assay precision was highly reproducible with coefficients of variance ranging from 0% to 4.7%. A comparison of 66 matched samples showed good agreement (R2=0.845) between the Altona and the reference laboratory assay, with an average negative bias (-0.24 log10 copies/mL). Genotyping analysis demonstrated that HAdV species B and C accounted for 77% of the positive samples. A significant (≥0.9 log10) difference in quantitation between both tests was found for three HAdV types (HAdV types A12, B14 and F41). In conclusion, the Altona RealStar® test is a reliable and sensitive assay for HAdV DNA quantitation.

publication date

  • December 2, 2017

Research

keywords

  • Adenovirus Infections, Human
  • Adenoviruses, Human
  • Polymerase Chain Reaction
  • Viral Load

Identity

Scopus Document Identifier

  • 85043506589

Digital Object Identifier (DOI)

  • 10.1016/j.diagmicrobio.2017.11.016

PubMed ID

  • 29433999

Additional Document Info

volume

  • 90

issue

  • 4