Growing Murine Bone Marrow-Derived Macrophages. Academic Article uri icon

Overview

abstract

  • Bone marrow-derived macrophages (BMDM) are primary macrophages obtained by in vitro differentiation of bone marrow cells in the presence of macrophage colony-stimulating factor (M-CSF or CSF1). They are easy to obtain in high yields, can be stored by freezing, and can be obtained from genetically modified mice strains. They are therefore widely used as prototypical macrophages for in vitro studies. In this chapter, we present the method for obtaining BMDMs and freezing them.

publication date

  • January 1, 2018

Research

keywords

  • Bone Marrow
  • Cell Culture Techniques
  • Macrophages

Identity

Scopus Document Identifier

  • 85047116141

Digital Object Identifier (DOI)

  • 10.1007/978-1-4939-7837-3_3

PubMed ID

  • 29761385

Additional Document Info

volume

  • 1784