A Robust Method for the Purification and Characterization of Recombinant Human Histone H1 Variants. Academic Article uri icon

Overview

abstract

  • Higher order compaction of the eukaryotic genome is key to the regulation of all DNA-templated processes, including transcription. This tightly controlled process involves the formation of mononucleosomes, the fundamental unit of chromatin, packaged into higher order architectures in an H1 linker histone-dependent process. While much work has been done to delineate the precise mechanism of this event in vitro and in vivo, major gaps still exist, primarily due to a lack of molecular tools. Specifically, there has never been a successful purification and biochemical characterization of all human H1 variants. Here we present a robust method to purify H1 and illustrate its utility in the purification of all somatic variants and one germline variant. In addition, we performed a first ever side-by-side biochemical comparison, which revealed a gradient of nucleosome binding affinities and compaction capabilities. These data provide new insight into H1 redundancy and lay the groundwork for the mechanistic investigation of disease-driving mutations.

publication date

  • January 8, 2019

Research

keywords

  • Histones
  • Protein Engineering
  • Recombinant Proteins

Identity

PubMed Central ID

  • PMC6541009

Scopus Document Identifier

  • 85060296214

Digital Object Identifier (DOI)

  • 10.1021/acs.biochem.8b01060

PubMed ID

  • 30585724

Additional Document Info

volume

  • 58

issue

  • 3