Detection of Zika virus in paired urine and amniotic fluid samples from symptomatic and asymptomatic women and their babies during a disease outbreak: association with neurological symptoms in newborns. Academic Article uri icon

Overview

abstract

  • Paired maternal and newborn urine and amniotic fluid from 138 subjects collected during a Zika virus (ZIKV) outbreak was analyzed for ZIKV by gene amplification (RT-qPCR), and the findings were correlated with clinical symptoms and neurological anomalies in the babies. ZIKV was detected in 1 of 9 symptomatic women (11.1%) and in 19 of 129 asymptomatic women (14.7%). Neurological manifestations were present in 19 babies (13.7%), 10 of 20 (50%) positive and 9 of 119 (7.6%) negative (p < 0.001) for ZIKV. Twelve (8.6%) urines collected during gestation were ZIKV-positive; only 2 remained positive for ZIKV postpartum. Six (4.1%) newborn urines collected within 1 day of delivery were ZIKV-positive cases. In 3 of these cases, ZIKV was detected in mother's urine pre- and postpartum and in both mother's urine and babies' urine. Four of the amniotic fluid samples (2.9%) were ZIKV-positive. Among ZIKV-negative babies with neurological sequel, 87.5% were female; in contrast, 72.7% ZIKV-positive babies with neurological abnormalities were male (p = 0.019). We conclude that during a ZIKV outbreak, clinical symptoms and ZIKV detection in biological fluids are poor predictors of infection and adverse neurologic sequel in newborns.

authors

  • Vedovello, Danila
  • Witkin, Steven S.
  • Silva, Andrea Cristina Botelho
  • Fajardo, Thamirys Cosmo Gillo
  • Mello, Alexandra Siqueira
  • Bertozzi, Ana Paula Antunes Pascalicchio
  • da Silva, Alify Bertoldo
  • Filho, Nemésio Florence Vieira
  • Rodrigues, Maria Manoela Duarte
  • Gazeta, Rosa Estela
  • Moron, Antônio Fernandes
  • Sarmento, Stéphanno Gomes Pereira
  • Soriano-Arandes, Antonio
  • Passos, Saulo Duarte

publication date

  • September 9, 2019

Research

keywords

  • Amniotic Fluid
  • Nervous System Diseases
  • Pregnancy Complications, Infectious
  • Zika Virus Infection

Identity

PubMed Central ID

  • PMC7040052

Scopus Document Identifier

  • 85073937250

Digital Object Identifier (DOI)

  • 10.1007/s13365-019-00797-0

PubMed ID

  • 31502209

Additional Document Info

volume

  • 26

issue

  • 1