Technical report: xMAPr - High-dynamic-range (HDR) quantification of antigen-specific antibody binding. Academic Article uri icon

Overview

abstract

  • Here, we demonstrate a high-dynamic-range quantification of antibody binding to single antigens in a multiplexed suspension bead array format. Using a dilution-based approach and the newly developed data analysis tool the quantitative dynamic range is increased by three orders of magnitude in the selected samples. The strong increase in dynamic range results in a more robust data basis for downstream analyses.

publication date

  • November 1, 2019

Research

keywords

  • Antibodies
  • Antigen-Antibody Reactions
  • Antigens
  • Blood Proteins
  • Fluorescent Dyes
  • Protein Array Analysis

Identity

Scopus Document Identifier

  • 85074800598

Digital Object Identifier (DOI)

  • 10.1016/j.jprot.2019.103577

PubMed ID

  • 31683064

Additional Document Info

volume

  • 212