CpG methylation in cell-free Epstein-Barr virus DNA in patients with EBV-Hodgkin lymphoma. Academic Article uri icon

Overview

abstract

  • Epstein-Barr virus (EBV) is associated with a variety of tumors and nonmalignant conditions. Latent EBV genomes in cells, including tumor cells, are often CpG methylated, whereas virion DNA is not CpG methylated. We demonstrate that methyl CpG binding magnetic beads can be used to fractionate among sources of EBV DNA (DNA extracted from laboratory-purified virions vs DNA extracted from latently infected cell lines). We then applied the technique to plasma specimens and showed that this technique can distinguish EBV DNA from patients with EBV-associated tumors (nasopharyngeal carcinoma, Hodgkin lymphoma) and viral DNA from patients without EBV-associated tumors, including immunocompromised patients and patients with EBV(-) Hodgkin lymphoma.

publication date

  • April 28, 2020

Research

keywords

  • Epstein-Barr Virus Infections
  • Hodgkin Disease

Identity

PubMed Central ID

  • PMC7189298

Scopus Document Identifier

  • 85084222894

Digital Object Identifier (DOI)

  • 10.1182/bloodadvances.2020001511

PubMed ID

  • 32311011

Additional Document Info

volume

  • 4

issue

  • 8