Chitinase 3-like 1 is neurotoxic in primary cultured neurons. Academic Article uri icon

Overview

abstract

  • Chitinase 3-like 1 (CHI3L1) is known to play a role as prognostic biomarker in the early stages of multiple sclerosis (MS), and patients with high cerebrospinal fluid CHI3L1 levels have an increased risk for the development of neurological disability. Here, we investigated its potential neurotoxic effect by adding recombinant CHI3L1 in vitro to primary cultures of mouse cortical neurons and evaluating both neuronal functionality and survival by immunofluorescence. CHI3L1 induced a significant neurite length retraction after 24 and 48 hours of exposure and significantly reduced neuronal survival at 48 hours. The cytotoxic effect of CHI3L1 was neuron-specific and was not observed in mouse immune or other central nervous system cells. These results point to a selective neurotoxic effect of CHI3L1 in vitro and suggest a potential role of CHI3L1 as therapeutic target in MS patients.

publication date

  • April 28, 2020

Research

keywords

  • Chitinase-3-Like Protein 1
  • Multiple Sclerosis
  • Neurons

Identity

PubMed Central ID

  • PMC7188887

Scopus Document Identifier

  • 85083975876

Digital Object Identifier (DOI)

  • 10.1016/j.jneumeth.2007.08.029

PubMed ID

  • 32346016

Additional Document Info

volume

  • 10

issue

  • 1