Quantitation of Secretory Granule Size in Drosophila Larval Salivary Glands. Academic Article uri icon

Overview

abstract

  • Maturation of secretory granules is a crucial process that ensures the bioactivity of cargo proteins undergoing regulated secretion. In Drosophila melanogaster, the larval salivary glands produce secretory granules that are up to four-fold larger in cross-sectional area after maturation. Therefore, we developed a live imaging microscopy approach to quantitate the size of secretory granules with a view to identifying genes involved in their maturation. Here, we describe the procedures of larval salivary gland dissection and sample preparation for live imaging with a fluorescence confocal microscope. Furthermore, we describe the workflow for measuring the size of secretory granules by cross-sectional surface area and statistical analysis. Our live imaging microscopy method provides a reliable read-out for the status of secretory granule maturation in Drosophila larval salivary glands.

publication date

  • June 5, 2021

Identity

PubMed Central ID

  • PMC8250388

Scopus Document Identifier

  • 85116190661

Digital Object Identifier (DOI)

  • 10.21769/BioProtoc.4039

PubMed ID

  • 34250205

Additional Document Info

volume

  • 11

issue

  • 11