Dipeptidyl Peptidase-4 from Cancer-associated Fibroblasts Stimulates the Proliferation of Scirrhous-type Gastric Cancer Cells. Academic Article uri icon

Overview

abstract

  • BACKGROUND/AIM: Cancer-associated fibroblasts (CAFs) may promote the malignancy of human scirrhous gastric cancer (SGC) cells. We conducted the present study to identify novel growth factors from CAFs. MATERIALS AND METHODS: OCUM-12 and 2 CAF cell lines were used. The proliferation of cancer cells was determined by the number of cancer cells or the MTT assay. The growth factor(s) were purified and characterized by the gel filtration chromatography and protein array. RESULTS: The molecular weight of the growth-stimulating factor was estimated to be approximately 66-669 kDa. Protein array of conditioned medium (CM) from CAFs indicated that dipeptidyl peptidase-4 (DPP-4) was one of the growth factors. The addition of CM increased the phosphorylation of C-X-C chemokine receptor 4 (CXCR4). The DPP-4 inhibitor significantly inhibited the growth-stimulating activity of CM. CONCLUSION: DPP-4 from CAFs might be one of the growth-stimulating factors for SGC through CXCR4.

publication date

  • January 1, 2022

Research

keywords

  • Adenocarcinoma, Scirrhous
  • Dipeptidyl Peptidase 4
  • Intercellular Signaling Peptides and Proteins
  • Receptors, CXCR4
  • Stomach Neoplasms

Identity

Scopus Document Identifier

  • 85121979292

Digital Object Identifier (DOI)

  • 10.21873/anticanres.15508

PubMed ID

  • 34969760

Additional Document Info

volume

  • 42

issue

  • 1