Immunohistologic dissection of the human kidney using monoclonal antibodies. Academic Article uri icon

Overview

abstract

  • Nine murine monoclonal antibodies which detect differentiation antigens of the human kidney are described. Immunofluorescence and immunoperoxidase studies demonstrate that these antigens are expressed by different cell types comprising the nephron. Monoclonal antibody MA99 detects a glycoprotein complex of the glomerular basement membrane. Monoclonal antibody S4 detects a glycoprotein of 160,000 daltons (gp160) expressed by glomerular and proximal tubular epithelial cells. Monoclonal antibodies S23, S27 and S6 immunoprecipitate a glycoprotein of 120,000 daltons (gp120) found on cells of the proximal tubule and portions of Henle's loop. Monoclonal antibody C26 identifies a glycoprotein of 40,000 daltons (gp40) expressed by cells of the distal and collecting tubules. Monoclonal antibodies M2 and S8 are specific for A and B blood group antigens, respectively, found on cells of the collecting tubule in individuals of the respective blood type. This panel of antibodies is useful in the study of normal renal embryogenesis, microanatomy and physiology as well as pathological processes including tumors.

publication date

  • March 1, 1985

Research

keywords

  • Antibodies, Monoclonal
  • Antigens, Surface
  • Kidney
  • Kidney Neoplasms

Identity

Scopus Document Identifier

  • 0021905727

PubMed ID

  • 3883008

Additional Document Info

volume

  • 133

issue

  • 3