Increased chromosomal instability in lymphocytes from elderly humans. Academic Article uri icon

Overview

abstract

  • Lymphocytes from young and old donors were incubated with PHA for 96 h and exposed to [3H]Tdr during the last 24 h of culture. Comparable amounts of [3H]Tdr were incorporated into chromosomes of old and young lymphocytes as measured by autoradiography of metaphase chromosomes. However, chromosomal damage and cell-cycle arrest were far greater in lymphocytes from old as compared to young humans. The frequency of chromosome breaks, fragments, exchange figures and dicentric chromosomes induced by [3H]Tdr was greater in cultures from old than in cultures from young humans. Lymphocytes from old donors exposed to 20 microM BrdU during the last 24 h of culture showed significantly more sister-chromatid exchanges than did lymphocytes from young donors. These data suggest that chromosomes in lymphocytes from old donors express more damage after exposure to [3H]Tdr or BrdU than do chromosomes in lymphocytes from young donors.

publication date

  • May 1, 1985

Research

keywords

  • Aging
  • Chromosomes
  • Lymphocytes
  • Sister Chromatid Exchange

Identity

Scopus Document Identifier

  • 0021962479

PubMed ID

  • 3990698

Additional Document Info

volume

  • 149

issue

  • 3