Assessing the Efficacy of Alcohol Swabbing in Preventing Bacterial Colonization of Single-Use Vials: A Pilot Study.

Overview

abstract

Introduction Proceduralists who use single-use vials have adopted many of the same techniques for sterilizing multi-use vials, including swabbing the rubber tops with 70% isopropyl alcohol before drawing injectate. However, the lack of literature specific to this sterilization technique for single-use vials brings into question the necessity of this practice. This pilot study aims to establish protocols and determine the feasibility of methods to assess the hypothesis that routine use of disposable alcohol swabs does not affect the risk of bacterial colonization of injectate vials. Methods Forty new injectate vials (n=20, vials with an aluminum cap; n=20, vials with a plastic cap) were randomly assigned to a "swab"/"no-swab" group. Vials assigned to the "swab" group were firmly swabbed once with 70% isopropyl alcohol after cap removal and allowed to air-dry. A blinded researcher sampled the exposed rubber stopper of the injectate vials by firmly swabbing the rubber with a sterile cotton culture swab dipped in sterile 0.9% normal saline. A microbiologist blinded to group assignment assessed the degree of bacterial growth from swabs of the injectate vials' rubber stoppers after incubation on blood agar plates. Results There was no growth in any of the 40 samples in either the "swab" or "no-swab" group on day 5 of plate incubation at 35°C in a CO2 environment. Subgroup analysis by vial cap material (plastic vs. aluminum) also revealed no detectable microbial growth in either group. Statistical testing confirmed no significant differences between groups (two-sided Fisher's exact test; P=1.00), and confidence intervals indicated a wide range of possible undetected effects due to limited power. These findings indicate that the swabbing of single-use vials prior to usage had no measurable impact on sterility under clean clinical conditions. Conclusion The findings of this pilot study provide valuable insights into the efficacy of routine alcohol swabbing in preventing bacterial colonization of single-use injectate vials. Despite the widespread practice of swabbing vial tops with 70% isopropyl alcohol before drawing injectate, the necessity of this protocol has been called into question due to the lack of specific literature supporting its effectiveness for single-use vials. However, our results demonstrate that neither swabbing nor the absence of swabbing resulted in bacterial growth on the rubber stoppers of injectate vials after two and five days of plate incubation. These findings suggest that for the types of vials assessed in this study, routine alcohol swabbing may not be essential for preventing bacterial contamination. Further research involving larger sample sizes and diverse types of injectate vials is warranted to confirm these findings and establish evidence-based guidelines for vial preparation protocols in clinical settings.