A microtiter solid phase radioimmunoassay for total serum IgE.
Academic Article
Overview
abstract
A two-step microtiter solid phase radioimmunoassay (MSPRIA) for total serum IgE has been developed by the authors. Microtiter wells are coated with rabbit antihuman IgE. During the first incubation. IgE in serum samples or the standard is bound to the antibody coated wells. The wells are then overlaid with radiolabeled goat anti-IgE antibodies. The amount of radioactivity bound is proportional to the amount of IgE in the test serum. The mean IgE level of 25 cord sera was found by this assay to be 0.3 U/ml. The total serum IgE's of 40 volunteers ranged from 4 to 593 U/cc. These values agreed with the values determined by commercial PRIST assay with a correlation coefficient of 0.98. The MSPRIA is a specific, sensitive, convenient and economical means of quantitating total serum IgE.
