Presence of point mutations in the N-ras gene in radiation-transformed rat embryo cells. Academic Article uri icon

Overview

abstract

  • We study the transforming ability of X-rays in multistep carcinogenesis by irradiating primary rat cells which contain transfected c-myc oncogene. X-irradiation induces fully transformed phenotypes, including anchorage-independent growth and tumor formation in nude mice. Of seven foci examined, five exhibited an A to G conversion in codon 61 of the N-ras oncogene. Another transformed isolate has a single G to A base substitution in codon 14 in the same oncogene, while no point mutation is detected in the other focus. This is the first in vitro demonstration of the association between point mutation and X-ray-transformed cells.

publication date

  • April 1, 1993

Research

keywords

  • Genes, myc
  • Genes, ras
  • Point Mutation

Identity

Scopus Document Identifier

  • 0027418611

PubMed ID

  • 8453615

Additional Document Info

volume

  • 53

issue

  • 7