Solubility study of albumin solders for laser tissue-welding. Academic Article uri icon

Overview

abstract

  • BACKGROUND/OBJECTIVE: Current albumin solders for tissue-welding are soluble in physiological fluids, prior to laser irradiation. These solders are therefore subjected to mechanical alterations, which can weaken the solder-tissue repair. In this study, an albumin solder (laser activated) was developed with low solubility and with the ability to retain (partially) its mechanical characteristics in saline solution. STUDY DESIGN/MATERIALS AND METHODS: Gauged protein samples of solder were immersed into 0.5 ml saline solution for fixed intervals of time. The solder samples contained four bovine serum albumin (BSA) concentrations: 56%, 66%, 70%, and 75% (by weight). A Bradford protein assay measured the BSA solubility of the solders. The 70% and 75% BSA solders were also used to weld in vitro Wistar rat intestine sections with a diode laser (lambda = 810 nm, power = 270 mW). RESULTS: The solubility of the 75% BSA solder was significantly decreased with respect to the other solders (Anova, P < 0.05). This solder also showed comparable weld strength (13 gm) to the 70% BSA solder. CONCLUSION: The 75% BSA solder strongly reduced the albumin solubility in saline solution, without affecting its tissue-welding properties.

publication date

  • January 1, 1998

Research

keywords

  • Laser Therapy
  • Serum Albumin, Bovine
  • Tissue Adhesives

Identity

Scopus Document Identifier

  • 0032422145

PubMed ID

  • 9888321

Additional Document Info

volume

  • 23

issue

  • 5