Immunolocalization of proliferating cells in the rabbit iliac artery after balloon angioplasty.
Academic Article
Overview
abstract
PURPOSE: Experiments were performed to characterize the location of proliferating cells in the balloon-dilated rabbit iliac artery. MATERIALS AND METHODS: Balloon angioplasty was performed on the external iliac arteries in each of four rabbits. The arteries were removed 3 days later, frozen, cryosectioned, and immunostained with Ki-67, an antibody that identifies proliferating cells. The sections were then examined to determine the patterns of cell proliferation within the arterial media and the ratio of proliferating to nonproliferating cells. RESULTS: Of the 31 arterial cross-sections examined, cell proliferation was circumferential in five (16%), and focal in 26 (84%). Of the 86 foci of proliferation examined within the 31 cross-sections, proliferation was localized to the inner media in 30 (35%), to the outer media in four (5%), and was transmural in 52 (60%). The internal elastica lamina (IEL) appeared normal at 22 foci (26%), but appeared stretched or torn at 64 (74%). Proliferation was usually confined to the inner media at foci having no IEL injury (18 of 22; 82%), but was most often transmural where the IEL was stretched or torn (49 of 64; 77%). The ratio of proliferating to nonproliferating cells, which averaged 0.31 +/- .20, was greater (P < .01) in areas with IEL injury than in areas without IEL injury. CONCLUSION: These results suggest that angioplasty-induced cell proliferation is typically focal rather than circumferential and is associated with stretching or tearing of the IEL.
