Shuttle vectors for genetic manipulations in Ustilago maydis. Academic Article uri icon

Overview

abstract

  • Shuttle vectors with new or improved features were constructed to enable facile genetic manipulations in the plant pathogen Ustilago maydis. Sets of plasmids selectable in media containing geneticin, carboxin, nourseothricin, or hygromycin, able to replicate autonomously, to transform U. maydis by integration, and to express foreign genes under control of the homologous glyceraldehyde-3-phosphate dehydrogenase promoter, were built upon a common pUC19 vector backbone. This permits a large number of choices for a cloning site, blue/white screening for recombinant plasmids, rapid transfer of a cloned DNA fragment between plasmids, and choice of several dominant drug-resistance markers for selection in U. maydis.

publication date

  • April 1, 2000

Research

keywords

  • Cinnamates
  • Genetic Vectors
  • Ustilago

Identity

Scopus Document Identifier

  • 0034067410

PubMed ID

  • 10779869

Additional Document Info

volume

  • 46

issue

  • 4